Hands-on Training on “Techniques in Enzymology” organized by the Department of Studies in Biotechnology

Purpose and Scope of the Event:

The hands-on training program on “Techniques in Enzymology”for seven days was conducted to provide M.Sc. Biotechnology students with in-depth practical exposure to fundamental and advanced methods used in enzymology. The primary objective of the program was to bridge theoretical knowledge with real-time laboratory experience by allowing students to perform experiments related to enzyme extraction, purification, kinetic analysis, enzyme activity assays, and evaluation of factors influencing enzyme function. The training aimed to enhance the technical competency of students, reinforce analytical and problem-solving skills, and prepare them for research-oriented academic and industrial careers. Through structured guidance and supervised practice, the program helped students gain confidence in handling enzymatic techniques, interpreting experimental results, and understanding the relevance of enzyme technology in biotechnology, biochemistry, pharmaceutical sciences, and diagnostics.

Details of the Talk/Event/Activity:

The hands-on training was conducted from 24th November 2025 to 01st December 2025 for M.Sc. Biotechnology students (1st and 3rd Semester), covering a total of 56 students divided into three batches. Each batch underwent two consecutive days of intensive laboratory training. The sessions were conducted by Dr. Girish Chandran, Assistant Professor & Course Coordinator, DoS in Biochemistry, School of Life Sciences.The hands-on sessions included demonstrations, guided practice, and supervised experimentation on:

• Extraction of intracellular and extracellular enzymes
• Techniques for enzyme purification (salt precipitation, dialysis, chromatography concepts)
• Enzyme kinetics (Michaelis-Menten analysis, Lineweaver–Burk plots)
• Determination of Vmax, Km, and specific activity
• Effect of pH, temperature, inhibitors, and activators on enzyme activity
• Preparation and standardization of enzyme assay reagents
• Interpretation and plotting of enzyme activity curves
• Data analysis and result interpretation

Students actively participated in pipetting, reagent preparation, spectrophotometric reading, graph generation, and troubleshooting experimental variations.

A Brief Profile of the Resource Persons:

Dr. Girish Chandran, Assistant Professor & Course Coordinator, Department of Studies in Biochemistry, School of Life Sciences, PBMMEC, Mysuru is Expert in enzymology, molecular biochemistry, and analytical techniques, Experienced researcher with contributions to enzyme kinetics, metabolic regulation, and protein biochemistry, Actively involved in curriculum development, laboratory training, and advanced skill-based workshops for postgraduate students.

Total No. of Participants/Beneficiaries: 56 M.Sc Biotechnology 1st & 3rd semester Postgraduate student

Glimpses of M.Sc Biotechnology – Batch I& Batch II& Batch III student’s with resource person Dr.Girish Chandran during the workshop