One day Hands on Workshop on High Performance Liquid Chromatography

Department of Studies in Biochemistry, School of Life Sciences,
Pooja Bhagavat Memorial Mahajana Post Graduate Centre,
PG Wing of SBRR Mahajana First Grade College, Mysore

The Workshop was organized with an objective to acquaint the students with knowledge of High-Performance Liquid Chromatography and its working principle, the program comprised of two sessions theory session in the Life Sciences auditorium and the practical session in R&D Centre.

The workshop coordinator was Dr. Girish Chandran, Assistant Professor, Coordinator DoS in Biochemistry. Pooja Bhagavat Memorial Mahajana Post Graduate Centre, PG Wing of SBRR Mahajana First Grade College, Mysore. In the first session Dr. Girish Chandran explained the basic principles of chromatography, High Performance Liquid Chromatography detectors with their applications, columns, binary and isocratic gradients, mobile phases, polarity and retention time. Function of UV-Visible detectors and Fluorescence detectors, their working, calibration and applications. Preparing test solutions, Mobile Phase, Setup method for High Performance Liquid Chromatography, Data acquisition software. Software Quantization, Integration and Report Generation. Method development, optimization of various chromatographic parameters and detailed the importance of specific applications, prevention, care, maintenance and trouble shooting. The resource person for the practical session was Ms. Rayana Lavitha D Silva. In the second session the students were taught to prepare the solutions and were allowed to work with HPLC. Initially the resource persons demonstrated how to handle and use the instrument step by step and helped the students to work with the High-Performance Liquid Chromatography system conveniently.

A brief profile of the resource persons

Dr. Girish Chandran
Assistant Professor, Coordinator DoS in Biochemistry, School of Life Sciences, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, PG Wing of SBRR Mahajana First Grade College, Mysore.
Qualification: M.Sc., (CSIR-JRF-NET), Ph.D. (Univ. of Mysore), Post Doc (USA)
Areas of Specialization: Drug development, small molecules, peptides, Nutritional interventions, Diet Therapy, quality foods, Neurodegenerative disorders, Pain, Behavioural aspects in Drosophila and Mice, Enzymology, Plant Biochemistry, Diabetes, Migraine, cancer, cross interactions drugs, comorbidity and therapy.

Ms. Rayana Lavitha D Silva
Assistant Professor, DoS in Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, PG Wing of SBRR Mahajana First Grade College, Mysore.
Qualification: M.Sc., (K-SET)
Areas of Interest: Neurodegenerative disorders, Cancer, Diabetes, Cardiovascular diseases and Analytical techniques.

The workshop was relevant to their course and was well-appreciated by the students. After completion of this workshop students have felt encouraged to explore further in analytical sciences. Target audience was B.Sc. students of SBRR Mahajana First Grade College and No. of Participants was 25.