Current Position: Assistant Professor & HOD
Qualification: M.Sc., Ph.D
Teaching Experience: 7 Years
Courses offered: Chemical Kinetics, Nuclear Chemistry, Catalysis, Electrochemistry, Organometallic Chemistry, Heterocyclic Compounds, Reagents, Chromatographic Techniques, Titrimetric Analysis, Phase rule and Statistical Thermodynamics.
Current research interests:

1. Synthesis of a novel biopesticide for pest control in food grain storage to minimize the hazards of chemical pesticides on human and environment.
2. Focused to work on Spectrofluorimetric and spectrophotometric assay for quantification of glucose and uric acid in human biological samples.
3. Developing new enzyme assay.
4. Computational work.

Doctoral Research:
My research area mainly focussed on the enzyme assay, method development for the quantification of different enzymes, enzyme kinetics and biological application. Important work done on the enzymes is:

1. Investigated method for quantification of glucose in human serum sample by coupling of 4-aminoantipyrine (4-AAP) and Iminodibenzyl (IDB) in the presence of bienzyme peroxidase (POD)/glucose oxidase (GOx ). The proposed method was applied for the quantification of glucose in human serum samples and results were comparable with the enzymatic kit method.
2. Developed simple and sensitive spectrophotometric assay for quantification of H 2 O 2 , on the basis of coupling of iso-nicotinicacidhydrazide (INH) and pyrocatechol (PC) as chromogenic co-substrates in presence of horseradish peroxidase (HRP) The proposed method has been applied in the estimation of H 2 O 2 in five different animals milk samples like goat, sheep, cow, buffalo and milk available in market using and rain water samples.
3. Established direct spectrophotometric assay for quantification of peroxidase activity in the presence of para-phenylenediamine dihydrochloride (PPDD) and 8-hydroxyquinolene (8-HQ) as chromogenic co-substrates. The developed method was successfully applied to study the effect of blanching treatment of peroxidase in sapota and fig fruit extracts.
4. Developed rapid method for determination of tyrosinase enzyme activity. The proposed method was applied to study the tyrosinase inhibition activity using mango seed extract.
5. Bienzymatic method developed for quantification of uric acid in human serum and urine samples.
6. Developed methods to study the antioxidant activity of some fruits, vegetables and spices.

Research Experience: 11 Years
Publications:
No. of publications: 10
No. of Papers presented in National/International symposia/conferences: 6

Research Publications:

1. Roopa R.A, Aradhana N, Mamatha M, Sarna Rani Bhagat, Chandresh B.S, Nagaraja P, 2014 “Colorimetric quantification of antioxidant capacity in some spices, vegetables and fruits extract”, International Journal of Innovative Pharmaceutical Sciences and Research, 2, 1915-1927.
2. Roopa R.A, Nagaraja P, 2015 “Quantification of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid mono sodium salt by oxidation with tyrosinase in the presence of 3-methyl-2-benzothiazolinone hydrazone”, Chemical Science Review and Letters, 4, 342-348.
3. Vindhya K, Pradeep Kumar P.M, Roopa R.A, Leelavathi S, 2015 “Evaluation of In-vitro antioxidant potential of various leaf extracts of Gardenia Latifolia Ait”, International Journal of Innovative Pharmaceutical Sciences and Research, 3, 176-190.
4. Roopa R.A., Mantelingu K, Rangappa K.S, Kumara M.N, 2016 “Development of Novel Bienzymatic Assay for Quantification of Glucose in Human Serum”, Asian journal of pharmaceutics, 10, S170-S176.
5. Roopa R.A. K. Mantelingu, K.S. Rangappa, 2016 “Evaluation of peroxidase assay and effect of thermal blanching on sapota and fig fruits”, Chemical data collection, 3–4, 46–57.
6. Roopa R.A. K. Mantelingu, Mridula Guin, 2017 ” Rapid and selective spectrophotometric assay for micromolar level determination of hydrogen peroxide based on biocatalysis of Horseradish peroxidase”, Analytical chemistry letters, 7, 779-791.
7. Roopa R. A and Mantelingu. 2019 “Spectrophotometric analysis of hydrogen peroxide with its applications illustrated by peroxidase assay”, Asian Journal of Research in Chemistry and Pharmaceutical Sciences, 7(2), 353-362.
8. Mridula Guin, Kamal Rautela, Roopa R.A, C.S. Shantharam, S. Begam Elavarasi, 2021 “Hydrogen bonded complexes of rhodanine with H2X/CH3XH (X = O, S, Se)”, Computational and Theoretical Chemistry 1196, 113134.
9. Roopa R A, K Mantelingu, Mridula Guin, Sridhar Bilgumba Thimmaiah, 2022 “Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine”, Journal of Analytical Chemistry, 77, 301–307.
10. Mridula Guin, Roopa R. A, Preeti Jain, Nakshatra Bahadur Singh, 2022 “Heterocyclic Surfactants and Their Applications in Cosmetics”, Chemistry Select, 7, e202103989 (1 of 25).

Book Chapter:

Book Name: Nanobiomaterials: Perspectives for Medical Applications in the Diagnosis and Treatment of Diseases
Chapter 3: Green Chemistry Principles and Spectroscopic Methods Applied to Nanomaterials Anindita De, Roopa R.A, Manasa H.S. Mridula Guin

List research papers presented in national and international conference:

1. Roopa R.A. 2013. “Rapid and selective spectrophotometric assay for micromolar level determination of hydrogen peroxide based on biocatalysis of Horseradish peroxidase”. National seminar on “Recent Advances in Chemical Biology – An Overview (RACE-2013)”, organized by the department of post graduate studies in chemistry, Government science college, Hassan, Karnataka,
2. Roopa R.A. 2014. “Quantification of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid mono sodium salt by oxidation with tyrosinase in the presence of 3-methyl-2-benzothiazolinone hydrazone”. National conference on “Recent Trends in Chemical Research” (NCRTCR-2014), held at Sri jayachamarajendra college of engineering, Mysore
3. Roopa R.A. 2014. “Development of novel bienzymatic assay for quantification of glucose in human serum”. International Symposium on “Chemical Biology – Drug Discovery” organized by University of Mysore,
4. Roopa R.A. 2014. “Evaluation of peroxidase assay and effect of thermal blanching on sapota and fig fruits”. “National Conference on Pure and Applied Chemistry” (NACOPAC-2014), organized by Department of Studies in Chemistry, University of Mysore, Mysore.
5. Roopa R.A. 2019. “Enzymatic determination of uric acid in human serum and urine using pyrocatechol and 3-methyl-2-benzothiazolinone hydrazone” University of Mysore, Mysore.
6. Roopa R.A. 2023. Bienzymatic Spectrophotometric Method for Uric acid Estimation in Human Serum and Urine”. International conference on Advances in Chemical and physical sciences, organized by department of science, amrita vishwa vidyapeetham, Mysore.